Cloning is wrong and should be outlawed by all becase of the moral wrongness of it. Ovine primary fetal fibroblasts were with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Ovine primary fetal fibroblasts were with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned and a population of neomycin (G 418) - resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were live born; three produced from cloned cells containing factor IX and neo trans genes, whereas three produced from the un cloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vito and produce viable animals by nuclear transfer.

Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear micro injection. Ovine primary fetal fibroblasts were with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned and a population of neomycin (G 418) - resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were live born; three produced from cloned cells containing factor IX and neo trans genes, whereas three produced from the un cloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vito and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear micro injection.

Ovine primary fetal fibroblasts were with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned and a population of neomycin (G 418) - resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were live born; three produced from cloned cells containing factor IX and neo trans genes, whereas three produced from the un cloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vito and produce viable animals by nuclear transfer.

Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear micro injection.