Chesapeake Bay Oysters For C Infection example essay topic

If you ever use this, please e-mail me at. I'd just like to know. Cryptosporidium: Transmission and Infection Cryptosporidium is a protozoan intestinal parasite causing a short-term enteric illness in individuals with functioning immune systems, and can cause a potentially fatal infection in immunosuppress ed individuals. Because of C.'s resistance to many of the procedures used to process drinking water and food, and the parasite's extremely high fecundity, the potential for a large scale outbreak is very high. In fact, C. was responsible for an outbreak in Milwaukee in 1993 when an estimated 403,000 people became ill.

This was the largest waterborne outbreak of disease in United States history. This paper will cover some aspects of C.'s life cycle, human sickness caused by the parasite, routes of transmission, and practices of control. There are six different species of Cryptosporidium, C. being the only species which infects mammals (Gutierrez, 1990). Oocysts, which are ingested by the mammal host, each contain four sporozoites. Upon encystment in the small intestine, the sporozoites infect an intestinal epithelial cell by becoming attached to the base of the micro villi. The sexual stages follow, where zygotes and eventually oo cysts are formed.

But C. also has an "auto-infecting" asexual stage in which thin walled oo cysts are produced to cause infection farther along in the intestine (Donnelly & Stentiford, 1997). Approximately 20% of the oo cysts produced will have these thin walls, leaving 80% of the oo cysts to be excreted out of the host and into the environment, where they will be infective immediately. Thirty oo cysts are enough to cause infection, and one infected person can excrete over a billion oo cysts in one day (Graczyk et al., 2000). Symptoms of Cryptosporidiosis in individuals include watery diarrhea (up to 3 liters a day), cramps, weight and appetite loss, nausea, vomiting and malaise (Gutierrez, 1990). Symptoms begin 3 to 5 days after the initial infection, and can last up to 2 weeks. Several relapses may occur due to the auto infecting mechanism of the parasite, but an otherwise healthy individual will rarely experience any more than 21 days of symptoms.

Oocysts, however, may continue to be shed in the host's feces for up to 2 months (Gutierrez, 1990). Because there is no known treatment for, symptoms in immunocompromised individuals may be much more severe. The infective dose may be as little as one oo cyst, and severe diarrhea can occur, causing the individual to pass up to 20 liters of fluid in one 24 hour period (Donnelly & Stentiford, 1997). This inevitably leads to death. Transmission of the parasite can occur in several different ways. Direct transmission can occur by handling infected animal or human feces.

One quarter of reported direct transmission infections occurred by direct contact with feces, while the rest were reported to have happened by person to person contact (Donnelly & Stentiford, 1997). Person to person transmission can occur through poor hygiene habits or by handling human waste. Day cares and nursing homes are at a high risk for person to person transmission because of the high risk of handling infected feces. Family outbreaks are common, as are outbreaks among children at nurseries (Donnelly & Stentiford, 1997). Indirect transmission by the water or food borne route is one of the most common ways C. is spread. Because of the oo cyst's resistance to chlorination, several outbreaks have been caused by waterborne transmission.

In one study (Carpenter et al. ), oo cysts were removed from the feces of an experimentally infected calf, cleaned of fecal matter, and placed into different amounts of chlorinated water at different temperatures. Although this experiment had been performed before, this was the first time that simulated recreational water was used. To simulate recreational water, the pH was balanced, Ca Cl 2 was added, as was organic material. The organic material was meant to simulate organic matter which might be found in a swimming pool, such as hair and feces. Feces is known to cause a negative effect on chlorine disablement of C. oo cysts. This is beneficial to the parasite because passage of the oo cysts occurs in the feces.

The experiment found that oo cysts maintained for three days at 200 C in 2 parts per million chlorine were still infective to laboratory mice. These conditions were meant to simulate recreational swimming pool conditions. This means that if an infected person were to release oo cysts in a swimming pool, other swimmers could become infected with the disease for up to three days. Contamination of water reserves is also an important factor in controlling potential outbreaks (Graczyk et al., 2000). Often lakes, rivers, streams and ponds surrounding livestock fields are contaminated with the parasite, which can cause contamination of a drinking water reserve. Testing for Cryptosporidium in a water reserve on a regular basis is not feasible due to cost, so an outbreak would very likely occur before the problem would be known.

Furthermore, some of the methods used to purify drinking water are not useful in controlling Cryptosporidium, so contamination of the source water would result in an outbreak. Several different factors would contribute to such contamination. An experiment was performed in Lancaster County, Pennsylvania to test different factors contributing to contamination (Graczyk et al., 2000). Digital maps of a particular floodplain were made showing all locations of cattle farms relative to the locations of water routes to and reserves of water (lakes, ponds, creeks and rivers). At 64% of the farmyards located within the floodplain, cattle were found to be infected with the parasite, and at 44% of the locations tested, oo cysts were found in all cattle samples.

DNA testing confirmed that the cattle downstream were infected with a "relative" of the parasite found in cattle upstream, indicating that the parasites were indirectly transmitted through the water Another experiment dealing with the susceptibility of a water system to environmental contamination of C. was carried out in Chesapeake Bay. This experiment used infected oysters for information. The objectives of this experiment were to find the most practical way of screening Chesapeake Bay oysters for C. infection, and to assess the role that other factors, such as cattle farms and wastewater treatment facilities, played in contamination of the Chesapeake Bay. After collecting a number of oysters from six different dredging sites, the oysters were tested for infection. Also waterfowl droppings from a number of sites near cattle farms were collected to test for the parasite. Use of a Mouse test and PCR were used to confirm that the oyster and waterfowl parasites were C...

All oysters collected had oo cysts within their hemocyte's. Oocysts were found in bird droppings at seven of the nine sites. The conclusions of this experiment is that due to the consumption of raw oysters from this area, there is the potential for a public health problem. The experiment also concluded that waterfowl are partially responsible for dispersing the parasite, as it would be possible for the birds to become infected several miles away from where they eventually deposit oo cysts into the environment in their feces (Graczyk et al., 2000). Foodborne transmission is another common way that infects humans.

Often infected fecal matter on the hands of the person preparing the food is the method of transmission. In one particular outbreak in 1995 in Minnesota (Anonymous, 1995), a daycare worker reportedly had handled diapers containing infected fecal matter, come into contact with oo cysts, and while preparing a chicken salad passed the disease to approximately 50 attendees of a social event. In conclusion it is important to remember that although C. is sometimes passed by food or by human to human contact, the source of major outbreaks, like that in Milwaukee in 1993, is by contamination of drinking water, making water treatment the obvious solution for control. Drinking water treatment, however, is difficult because the oo cysts are resistant to many of the methods used for purification, including chlorination. Areas where reservoirs for the parasite exist (i.e. livestock, oyster beds and migratory birds) are also at a higher risk of epidemics of C... Members of the AIDS community are at more risk than others of contracting a severe infection due to their compromised immune systems, and everyone is at risk of obtaining the parasite through its water, food, or human borne routes of transmission.

Immediate action by Federal and state environmental agencies should be taken to avoid a large scale outbreak. Such action could include more frequent testing of water supplies and reserves for oo cysts, and plans for containment in the event of an outbreak. Education of the public should also occur so that symptoms are reported in a timely manner to authorities so that, in case of an outbreak, measures could be taken to prevent further infection.

Bibliography

Donnelly, J.K., Stentiford, E.I. (1997) "The Cryptosporidium Problem in Water Food Supplies" Lebens m.
Wiss. U. -Technol., 30,111-120 Graczyk, T.K., Evans, B.M., Shift, C.J., Karr eman, H.J. Path, J.A. (2000) "Environmental and Geographical Factors Contributing to Watershed Contamination with Cryptosporidium Oocysts" Environmental Research Section A 82,263-271 Graczyk, T.
K., Fayer, R., Trout, J.M., Jenkins, M.C., Higgins, J., Lewis, E.J. Farley, C.A. "Susceptibility of the Chesapeake Bay to Environmental Contamination with Cryptosporidium " Environmental Research Section A 82,106-112 Carpenter, C., Fayer, R., Trout, J., Beach, M.J., "Chlorine Disinfection of Recreational Water for Cryptosporidium " CDC Dispatch -- - web Yez id (1990) "Diagnostic Pathology of Parasitic Infections with Clinical Correlations" 94-107.