Enzyme Activity example essay topic
300 words
Enzyme are biological catalyst to speed up a specific chemical reaction by providing an alternative pathway with the lower activation energy. Enzyme are protein in nature, it has its own 3-dimension configuration. It works due to presence of active site, a specific region with specific configuration, are able to bind with substrate to form enzyme-substrate complex for reaction. The specific 3-dimension configuration is held by bonds within enzyme. However, heat affect the enzyme activity. Heat provides kinetic energy to both substrate and enzyme melocules, increases the chance of effective collisions between subst arte and enzyme molecules then then enzyme activity raise.
When vibrations become too violent's, chemical bonds in enzyme molecules break and precise 3-dimensional structure, especially for active site, the function of enzyme loss. Enzyme is denatured. These all alter the enzyme activity. At to low temperature, the enzyme moelcules and substrates do not have enough kinetic energy to do collision, the enzyme activity also is low. To study the effect of temperature, amylase is used. Amylase digest starch to simple sugar, maltose and glucose.
Starch is a mixture of two polysaccharides, which are react with iodine to form a characteristic blue-black colour of standard iodine test. However, when a starch was disgusted to a mixture of simple sugars, the mixture does not react with iodine to give blue-black solution. The brown colour of iodine can been seen. After the experiment, it shows that the amylase activity increases from 0 c to 60 c, and at 60 c it has a maxim an activity rate, after that the activity rate decreases til 0 c. It shows that the opium temperature of amylase is 60 c and it would be denatured around 100 c.
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