Move The Same Distance From The Wells example essay topic

Title: Principles and Practice of Agarose Gel ElectrophorsisObjectives: To detect the size, shape and charge of the each dye solution. Methods: Casting the Agarose Gel In this experiment. 8% solution was used. By using a 250 ml flask the buffer solution was prepared.

Using the equation to make enough solution for the in tire lab class the equation had to be multiply ed by five. The contents of this equation were added to the 250 ml flask and swirled to evenly distribute it contents. Then a mark was placed on the outside of the flask to indicate the level of the solution before heating. The flask opening had pera film placed over it so that there was little to no evaporation. The solution was then placed in the microwave and heated. The solution was then heated for one min and sw riled for evenly dissolved aga rose.

The aga rose was then cooled, so that it was not to hot and the plate would crack. Some water was added to the solution because of there was some evaporation during heating. Once the gel had cooled, it was poured into the plate between the rubber dams. The plate was filled about half way up the comb arms. These dams are placed in the plate to prevent leaking. Then the gel was added and allowed to, which takes around 20 mind.

Preparing the Gel for Electrophoresis Once the rubber dams have been removed (carefully), the comb was then removed. Then the buffer was made. The buffer was made by using the equation, but also it by five, for the five lab groups. Then the chambers around the gel plate is filled with the buffer, just enough buffer to cover the gel plate in a very small amount. Then the dyes were loaded to there correct wells.

Once the gels were added (carefully) the lid was placed on the plate and system was turned on. The system ran for about 10 mind. (Hint the system is running when there are bubbles occuring in the buffer solution.) Results Look at attached page for diagram of the gel. The results indicate that the orange dye was the smallest of the dyes. This is because it traveled the farthest from the wells. The purple and the practice dyes were the same size because they both move the same distance from the wells.

The red dye is shown to be heavy er then all the dyes so further, but not as heavy as the blue dyes. The Blue is the heaves t dye because it move a very short distance from the wells. When the Blue 1 and the Blue 2 were mixed and placed in the same well they moved the same amount of distance away from the wells but they moved in opposite directions. Which means that one is positive and one is negatively charged. The blue 1 negatively charged because it moves toward the positive side of the plate, were as with blue 2 is charged because it move toward the negative side of the plate.

Also the mixture is very long because it shows all the dyes moving though the gel. Questions 1) The aga rose gel separates based on the size, shape and charge of the molecule. 2) The molecule will migrate toward the the opposite pole. Like if he molecule is positive it will move toward the negative pole, and if the molecule is negative it will move toward the positive pole. 3) It can be concluded that F (Blue 1 and Blue 2) are the same shape and size because they moved the same distance from the wells. But they have opposite chargers because they move to different poles.

4) This makes the solutions have a greater denis ty. So there will be more act urate results. 5) The experiment would not work because there are items in water that would cause the dyes to have moving in the gel..