Tick Gut Protein example essay topic

CATTLE TICK Nature of the disease The cattle tick Boophilus microplus is a significant ectoparasite of cattle and a vector for important diseases such as babesiosis and anaplasmosis. Classification SPC List D disease Susceptible species B. microplus is primarily a parasite of cattle. However, heavy infestations can also occur on horses, sheep, deer and water buffalo. Rarely, ticks have been reported on marsupials, goats, dogs, cats and pigs. Where it occurs The cattle tick is widely distributed in Central and South America, parts of the southern USA, Africa, Asia, and northern Australia. The distribution of the cattle tick is largely determined by climatic factors. B. microplus requires high humidity and ambient temperatures of at least 15-20 o C for egg laying and hatching.

Clinical signs Cattle tick infestation causes: damage to hides loss of production anaemia and death weakness leading to greater mortalities during droughts Post-mortem findings Animals may be in poor condition, anaemic. Infestations will be obvious by the presence of engorged ticks attached to the animal's skin. Differential diagnosis Several species of ticks may be found on cattle and it is important to differentiate these from B. microplus. Other ticks include: Haemophysalis sp... Ixo des holocyclus Specimens required for diagnosis Ticks should be collected and forwarded to an entomologist for confirmation of the diagnosis. B. microplus has pale legs, short mouth parts with transversely ridged palms, small eyes and lacks an anal groove.

Transmission The life cycle involves free-living stages. After feeding on cattle, engorged female ticks drop to the ground and lay eggs (up to 5000). After hatching, the larvae survive on pasture for sever a months. The larvae then become quite active and climb up grass and transfer to animals as they brush past. The larvae attach and feed from the host. They moult to the nymphal stage and then undergo a further moult to the adult stage.

Risk of introduction Cattle ticks are most likely to be introduced with the importation of infested cattle. Cattle tick was introduced to New Caledonia with cattle imported from Australia in this way. Transport of ticks on dogs is considered a potential risk for introducing the tick to new areas, although egg production is much reduced in this species and the risk is considered low. Control / vaccines There are four methods available for controlling ticks: 1. treatment with acaricides 2. pasture spelling 3. the use of resistant cattle 4. vaccination Tick control by acaricide dipping has been widely used in endemic areas. Acaricides used for this purpose include various synthetic pyrethroids, amit raz, and some organophosphate's. Dipping may have to be done as frequently as every 4-6 weeks in heavily infested areas.

Many producers in tick endemic area have changed to Bos indic us type cattle because of their greater resistance to tick infection. An anti-tick vaccine is also commercially available in Australia. The vaccine antigen, based on a tick gut protein and produced by recombinant technology, stimulates production of specific antibodies in cattle which damage the gut of engorging ticks, resulting in a fertility reduction of up to 70% in adult ticks, reducing tick population build-up. This vaccine is of limited use, but can be used as part of an integrated program for the control of ticks.

Each year 150 million dollars is lost to Australia's beef and dairy industries because of cattle tick infecting our cattle. Cattle ticks are ecto-parasites which live in the coat of the cattle and suck their blood. By doing this, many diseases like tick fever can be transferred causing poor health, and the effects can be fatal. Tick fever is a malaria-like disease and is described by Peter Willadsen as having produced "one of the biggest disasters in our agricultural history".

It is not unusual for 1000 female ticks to be found on one cow at a time. Together they can suck more than a litre of blood in one day. The female ticks then drop to the ground to reproduce producing another cycle of ticks to infest cattle. Control for diseases caused by these ticks has to be continually kept under control. Right now, farmers immerse their cattle in toxic chemicals to get rid of the ticks. However, this raises the issue of having these toxic chemicals remain in the beef, and having toxic waste in the environment.

More alarming, cattle ticks have an amazing history of becoming resistant to the chemicals used to kill them. It is only a matter of time before the ticks become resistant to the chemicals being used today. Due to this, a new vaccine called TickGARD (which is based on the BM 86 protein from the ticks' gut) is being introduced. When the tick tries to feed off the cow by 'sucking its blood' the inside of the tick becomes confused when the blood from the cow is recognised as blood from a tick due to the use of TickGARD for the cow. The walls retract and the blood is released into the rest of the tick including the arms and the legs. This method cuts down their survival rate and most importantly makes it less likely for the tick to reproduce.

It does not eradicate the ticks but they do become severely impaired and have a much shortened and more difficult life span due to this. Peter Willadsen has said 'We estimate an overall reduction of up to 70% in tick fertility using TickGARD. In an integrated control program it should cut the need for chemical treatments by between two and three applications per season. ' Genetic engineering is required for the vaccine because to produce Tickgard around 20,000 ticks are needed.

This is a difficult demand to keep up with and so instead of breeding the cattle ticks the traditional way the ticks are genetically engineered as this is a faster easier method. A specific antigen is needed to be found and isolated from samples which are taken from the gut of a tick. The antigen needed was a protein which triggers the immunity sytem. To isolate the particular protein it had to be identified from about ten thousand proteins found in the gut.

Once the particular gene is isolated from the tick it is put into the bacterium which then produces a synthetic antigen. Lastly, this synthetic antigen is inserted into a stable vaccine..